Journal: iScience

Article Title: Immunometabolic adaptation in monocytes underpins functional changes during pregnancy

doi: 10.1016/j.isci.2024.109779

Figure Lengend Snippet:

Article Snippet: CD163 human antibody (clone GHI/61.1) , Miltenyi , Cat#130-123-249; RRID: AB_2819455.

Techniques: Recombinant, Adhesive, Sequencing, Modification, DC Protein Assay, Staining, Imaging, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Gene Expression, Software, Saline

Journal: bioRxiv

Article Title: Distinct roles for thymic stromal lymphopoietin (TSLP) and IL-33 in experimental eosinophilic esophagitis

doi: 10.1101/2025.02.25.640192

Figure Lengend Snippet: Experimental EoE was induced in wild type (WT) mice using oxazolone (OXA). Starting on day 19, the mice received two intraperitoneal injections per week of isotype control antibodies or anti-TSLP (clones M702 or clone 28F12, A). On day 36, the mice were euthanized, and esophageal tissues were fixed, paraffin embedded, and slides were generated. The slides were stained with anti-major basic protein (MBP, B) and esophageal eosinophils were quantified (C). Epithelial cell proliferation was determined using anti-Ki67 staining (D, E). H&E-stained slides (G) were analysed for lamina propria (F) as well as epithelial (H) thickness. Esophageal vascularization was determined using anti-CD31 staining (I, J). Single cell suspensions of esophageal tissue were obtained from oxazolone-challenged isotype control or anti-TSLP (M702)-treated mice using enzymatic digestion and the levels of eosinophils and CD4 + T cells determined by flow cytometry (K, L). Representative photomicrographs of anti-MBP (B), anti-Ki-67 (D), H&E (G) and anti-CD31 (I) are shown. Data are presented as mean ± SEM and are representative of n=3 experiments conducted with 10-12 mice per group, ns-nonsignificant, *-p<0.05, **-p<0.01, ***-p<0.001, ****-p<0.0001 .

Article Snippet: To substantiate these findings, TSLP was neutralized using an additional clone of anti-TSLP (BioXcell, clone 28F12).

Techniques: Control, Clone Assay, Generated, Staining, Flow Cytometry

Journal: bioRxiv

Article Title: Distinct roles for thymic stromal lymphopoietin (TSLP) and IL-33 in experimental eosinophilic esophagitis

doi: 10.1101/2025.02.25.640192

Figure Lengend Snippet: Experimental EoE was induced in wild type (WT) mice. Starting on day 19, the mice received two intraperitoneal injections per week of isotype control antibodies or anti-TSLP (clones M702 or clone 28F12). On day 36, the mice were euthanized, and esophageal tissue was obtained and subjected to bulk RNA sequencing. PCA plot of vehicle- and oxazolone (OXA)-challenged mice treated with either isotype control or anti-TSLP antibodies (clones M702 and/or 28F12) is shown (A). Heat plot analysis of all differentially expressed genes (absolute fold change > 2, p<0.05) (B) is presented. Analysis of the top differentially expressed secreted factors, cytokine receptors, adhesion molecules and extracellular matrix components is shown (C-D). Gene Set Enrichment Analysis (GSEA) and STRING analysis were performed on the DEGs, and comparison of OXA-challenged vehicle-treated mice vs. OXA-challenged anti-TSLP treated mice was performed (E-H). The top up and down regulated pathways, which were regulated by TSLP are presented (E-F). In C, D and E, each lane represents a different mouse.

Article Snippet: To substantiate these findings, TSLP was neutralized using an additional clone of anti-TSLP (BioXcell, clone 28F12).

Techniques: Control, Clone Assay, RNA Sequencing, Comparison